BIODEGRADATION OF USED BABY DIAPERS USING CELLULOLITIC FUNGUS AND BACTERIA WITH SOLID FERMENTATION

  • Riryn Novianty University of Riau
Keywords: Bacteria, cellulase, solid fermentation, Trichoderma asperellum, used diaper

Abstract

Diapers are made by cotton and pulp containing cellulose that can be used as a substrate in the production of cellulose enzymes. The purpose of this research is to determine the ability of cellulolytic fungi Trichoderma asperellum LBKURCC1 and cellulolytic bacteria S-22 to degrade the used diapers containing urine by solid fermentation for 10, 20, and 30 days. The activity of crude extract of cellulose enzyme was observed with CMC 2% as substrate at pH 5,5 (Trichoderma asperellum LBKURCC1) and pH 7 (bacteria S-22 isolate), incubation temperature 40ºC during 30 minutes by using Nelson Somogyi’s method. The result showed that used diapers can be used as substrate for cellulose enzyme production by fungi Trichoderma asperellum LBKURCC1 with the highest activity of cruide extract cellulose enzyme obtained at 10th days solid fermentation of (1,891 ± 1,453) × 10-3 U / mL and by bacteria S-22 isolate at 20th days solid fermentation of (2,854 ± 0,019) × 10-3 U/mL. It can be concluded that bacteria  S-22 isolate would degrade the used diapers better than fungi Trichoderma asperellum LBKURCC1. The result of this research compared to quality standards compost of SNI: 19-7030-2004 have not fulfilled, so it can not be used as compost.

References

1. Aminah, S., Soedarso, G.B., Sastro, Y. 2003. Teknologi Pengomposan. Balai Pengkajian Teknologi Pertanian, Jakarta.
2. Acharya, P.B., Acharya & Modi, H. A. 2008. Optimazation for cellulose production by Aspergillus niger using saw dust as substrate. African journal of biotechnology. 22: 4147-52.
3. Badan Pusat Statistik. 2013. Proyeksi penduduk indonesia 2010-2035. katalog BPS: 2101018. Badan Pusat Statistik, Jakarta. Tersedia di: http://www.bps.go.id/index.php/publikasi/16. Tanggal akses 12 juni 2017.
4. Chalimatus, H., Latifah, Mahatmanti, F.W. 2013. Efektifitas Jamur Trichoderma harzianum dalam Pengomposan Limbah Sludge Pabrik Kertas. Indonesian Journal of Chemical Science 2(3): 224-229.
5. Cowd, M.A. 1991. Kimia Polimer. Penerbit ITB, Bandung.
6. Damayanti, N. 2015. Biodegradasi popok bayi menggunakan jamur selulolitik Trichoderma asperellum LBKURCC1 dan LBKURCC2 dengan fermentasi. Skripsi. FMIPA Universitas Riau, Pekanbaru.
7. Doraja, P.H. Shovitri, M., Kuswytasari, N.D. 2012. Biodegradasi limbah domestik dengan menggunakan inokulum alami dari tangki septik. Jurnal Sains Dan Seni ITS. 1(1) : 44-47.
8. Djunarnani,N. Kristian, Setiawan, B.S. 2012. Cara cepat membuar kompos. Agromedia pustaka. Jakarta.
9. Indriani, H.Y. 2011. Membuat Kompos Secara Kilat. Penebar Swadaya, Jakarta.

10. Koto, R.G. 2013. Kecernaan in vitro bahan kering dan bahan organik jerami jagung (Zea Mays) yang di inokulasi dengan Trichoderma sp. pada inkubasi yang berbeda. Skripsi. Fakultas Pertanian Universitas Hasanuddin, Makassar.
11. Lestari, P.B., Hartati, T.W. 2017. Mikologi Berbasis Inkuiri. Penerbit Gunung Samudera, Malang
12. Maniatis, T., F.Frictch, Sambrook. 1989. Molecular Cloning.Cold spring harbor laboratory press, New York.
13. Nugroho, T.T., Ali, M., Ginting, C., Wahyuningsih, Dahliaty, A., Devi, S., Sukmarisa, Y. 2003. Isolasi dan karakterisasi sebagian kitinase Trichoderma viride TNJ63. Jurnal Natur Indonesia 5(2): 101-106.
14. Satyawiharja, B. 1984. Fermentasi Media Padat dan Manfaatnya. Dirjen Dikti, Jakarta.
15. Siregar S.A. 2005. Instalasi Pengolahan Limbah. Penerbit Kanikus, Yogyakarta.
16. Suhartono, M. T. 1989. Enzim dan Bioteknologi. IUC-Bank Dunia XVII, Bogor.
17. Sulaeman., Suparto., Eviati. 2005. Analisis Kimia Tanah, Tanaman, Air, dan Pupuk. Balai Penelitian Tanah, Jakarta.
18. Supadma, A.A.N Arthagama , & D.E. 2008. Uji formulasi kualitas pupuk kompos yang bersumber dari sampah organik dengan penambahan limbah ternak ayam, sapi, babi dan tanaman pahitan.Jurnal Bumi Lestari, 8 (2): 113-121.
19. Suwandi.2009. Menakar Kebutuhan Hara Tanaman Salam Pengembagan Inovasi Budaya Sayuran Berjelanjutan. Inovasi Pertania. Jakarta.
20. Sharhanis, A., dkk. 2011. Sustainability Assesment : Seventh Generation Diapers versus gDiapers. The University of Vermont, Burlington.
21. Tobing, E.L. 2009. Studi tentang kandungan nitrogen, karbon organik dan C/N dari kompos tumbuhan kembang bulan (Tithonia diversifolia). Skripsi. FIMIPA USU.
22. Yuliprianto, H. 2010. Biologi Tanah dan Strategi Pengolahannya. Graha Ilmu, Yogyakarta.
23. Yunasfi. 2008. Serangan Patogen dan Gangguan Terhadap Proses Fisiologi Pohon. Universitas Sumatera Utara, Medan.
24. Yuwono, T. 2005. Biologi Molekuler. Erlangga, Jakarta.9
25. Wijaksono R.A., Subiantoro R., Utoyo B. 2016. Pengaruh lama fermentasi pada kualitas pupuk kandang kambing. Jurnal Agro Industri Perkebunan. Politeknik Negeri Lampung, Bandar Lampung.
Published
2020-04-29
How to Cite
Novianty, R. (2020). BIODEGRADATION OF USED BABY DIAPERS USING CELLULOLITIC FUNGUS AND BACTERIA WITH SOLID FERMENTATION. JURNAL INOVASI PENDIDIKAN DAN SAINS, 1(1), 13-19. Retrieved from http://ejournal.unwmataram.ac.id/JIPS/article/view/228
Section
Artikel